检索范围:
排序: 展示方式:
Kishore Gopalakrishnan, Javad Roostaei, Yongli Zhang
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1075-2
RSM is used to explore the impact of different parameter on algal growth response. Mixed algal culture promotes algal biomass and lipid accumulation. Optimized conditions achieve maximum productivity of algal biomass and lipid.
关键词: Algal biofuels Algal mixed cultures Algal biomass Algal lipid Wastewater Response surface methodology
Mingyu LIN,Yao Hsiang TSENG,Chin-Pao HUANG
《化学科学与工程前沿(英文)》 2015年 第9卷 第2期 页码 242-257 doi: 10.1007/s11705-015-1513-7
关键词: nano-TiO2 Pseudokirchneriella subcapitata algal cells titanium dioxide uptake
Algal biomass derived biochar anode for efficient extracellular electron uptake from
Yan-Shan Wang, Dao-Bo Li, Feng Zhang, Zhong-Hua Tong, Han-Qing Yu
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1072-5
Algal biochar anode produced higher biocurrent compared with graphite plate anode. Algal biochar exhibited stronger electrochemical response to redox mediators. Algal biochar showed excellent adsorption to redox mediators.
关键词: Algal biochar Anode material Electrochemical activity Extracellular electron transport Waste resource utilization
《农业科学与工程前沿(英文)》 2023年 第10卷 第4期 页码 566-578 doi: 10.15302/J-FASE-2023520
Elucidating the spatiotemporal pattern of water quality and algal biomass is crucial for accurately tracing pollution sources and reducing the risk of algal blooms in lake systems. This study analyzed the spatiotemporal variability of water quality and algal biomass in Erhai Lake from 1994 to 2021 using water quality index (WQI), Mann-Kendall test and Sen’s slope combined methods. The potential causes of water quality deterioration and algal biomass dynamics were also elucidated. The results showed that the historical changes in the water environment of Erhai Lake mainly had three stages: satisfactory (1994‒2001), deteriorating (2002‒2010) and improving (2011‒2021). The changes in water quality and algal biomass were primarily affected by total nitrogen, total phosphorus and chemical oxygen demand in different stages. The water environment of Erhai Lake is currently improving significantly, starting in the southern area that is furthest from the sources of agricultural pollution, especially in summer and autumn. This is attributed to the implementation of control measures resulting in lower pollutant loads at particular times and places. Therefore, it is necessary to continue to promote standardized livestock farming, to strengthen rural wastewater collection and to investigate measures such as the interruption of the endogenous cycle.
关键词: Erhai Lake control measures water environment water quality index (WQI)
Removing nitrogen and phosphorus from simulated wastewater using algal biofilm technique
WEI Qun, HU Zhiquan, LI Genbao, XIAO Bo, SUN Hao, TAO Meiping
《环境科学与工程前沿(英文)》 2008年 第2卷 第4期 页码 446-451 doi: 10.1007/s11783-008-0064-2
《环境科学与工程前沿(英文)》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1564-1
● A new model for bloom control in open land scape water was constructed.
关键词: Reclaimed water landscape Algal bloom Nitrogen and phosphorus Hydraulic retention time Threshold Control model
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 8-27 doi: 10.15302/J-FASE-2018233
Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.
关键词: embryonic germ cells embryonic stem cells induced pluripotent stem cells pigs pluripotent stem cells
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
《医学前沿(英文)》 2007年 第1卷 第3期 页码 294-298 doi: 10.1007/s11684-007-0056-9
Innate and adaptive T cells in influenza disease
null
《医学前沿(英文)》 2018年 第12卷 第1期 页码 34-47 doi: 10.1007/s11684-017-0606-8
Influenza is a major global health problem, causing infections of the respiratory tract, often leading to acute pneumonia, life-threatening complications and even deaths. Over the last seven decades, vaccination strategies have been utilized to protect people from complications of influenza, especially groups at high risk of severe disease. While current vaccination regimens elicit strain-specific antibody responses, they fail to generate cross-protection against seasonal, pandemic and avian viruses. Moreover, vaccines designed to generate influenza-specific T-cell responses are yet to be optimized. During natural infection, viral replication is initially controlled by innate immunity before adaptive immune responses (T cells and antibody-producing B cells) achieve viral clearance and host recovery. Adaptive T and B cells maintain immunological memory and provide protection against subsequent infections with related influenza viruses. Recent studies also shed light on the role of innate T-cells (MAIT cells, gd T cells, and NKT cells) in controlling influenza and linking innate and adaptive immune mechanisms, thus making them attractive targets for vaccination strategies. We summarize the current knowledge on influenza-specific innate MAIT and gd T cells as well as adaptive CD8+ and CD4+ T cells, and discuss how these responses can be harnessed by novel vaccine strategies to elicit cross-protective immunity against different influenza strains and subtypes.
关键词: influenza innate T cells CD4+ and CD8+ T cells vaccination
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 28-32 doi: 10.15302/J-FASE-2018222
Primordial germ cells (PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by first dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells (iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established. Here, we discuss recent advances in the stem cell field for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.
关键词: pig pluripotent stem cells primordial germ cells reprogramming
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 20-25 doi: 10.1007/s11684-009-0006-9
关键词: 4-1BB ligand tumor immunotherapy tumor microenvironment
The past, present and future of bovine pluripotent stem cells: a brief overview
Xiuchun TIAN
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 3-7 doi: 10.15302/J-FASE-2018247
Although the pursuit of bovine embryonic stem cells started more than 26 years ago for the purpose of gene-targeting, true pluripotent stem cells in this economically important species are still elusive. With the rapid advances in genome-editing and cloning using homologously recombined somatic cells, the need for pluripotent stem cells for precise genetic modification in any species became questionable. With the pig being the better model for human regenerative biology, the identification of the commonalities and uniqueness of the pluripotency circuitry across mammalian species may be the main objective for studying pluripotent stem cells in the bovine.
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
Algal-bloom control by allelopathy of aquatic macrophytes-A review
HU Hongying, HONG Yu
《环境科学与工程前沿(英文)》 2008年 第2卷 第4期 页码 421-438 doi: 10.1007/s11783-008-0070-4
关键词: development convenient alternative inhibitory application allelopathic
Yu Liu, Qiao Zhang, Yu Hong
《环境科学与工程前沿(英文)》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0938-2
关键词: Chlorella sp. HQ Chlorination Disinfection byproducts Fluorescence spectroscopy Soluble algal products
标题 作者 时间 类型 操作
Algal biomass derived biochar anode for efficient extracellular electron uptake from
Yan-Shan Wang, Dao-Bo Li, Feng Zhang, Zhong-Hua Tong, Han-Qing Yu
期刊论文
SPATIOTEMPORAL VARIATION OF WATER QUALITY AND ALGAL BIOMASS IN ERHAI LAKE AND ITS ENVIRONMENTAL MANAGEMENT
期刊论文
Removing nitrogen and phosphorus from simulated wastewater using algal biofilm technique
WEI Qun, HU Zhiquan, LI Genbao, XIAO Bo, SUN Hao, TAO Meiping
期刊论文
Modelling the thresholds of nitrogen/phosphorus concentration and hydraulic retention time for bloom control in reclaimed water landscape
期刊论文
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
期刊论文
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
期刊论文
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
期刊论文
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
期刊论文
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
期刊论文